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I am currently trying to use local symmetry in relion.
I follow the information provided in the wiki.Unfortunately I am stuck at the first step (global search of operators).
I am currently trying to use local symmetry in relion.
I follow the information provided in the wiki.Unfortunately I am stuck at the first step (global search of operators).
relion_localsym --search --i_map 3D-final.mrc --i_op_mask_info init_masklist.star --o_mask_info maskinfo_iter000.star --angpix 1.24 --ang_step 5
the error message I get:
ERROR:
ERROR: Cannot open file: maskinfo.txt
File: /home/gerald/relion/src/local_symmetry.cpp line: 209
My file init_masklist.star
data_
loop_
_rlnMaskName #1
_rlnAreaId #2
mask-t1-m1.mrc 1
mask-t1-m2.mrc 1
mask-t1-m3.mrc 1
mask-t2-m1.mrc 2
mask-t2-m2.mrc 2
mask-t2-m3.mrc 2
I checked with chimera that the masks provided are aligned with the 3D (same box size, etc)
Best
Rémi
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