diff --git a/packages/vite/src/node/plugins/workerImportMetaUrl.ts b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
index 0a7b34d4ff3dc8..3b0fdfb1e97548 100644
--- a/packages/vite/src/node/plugins/workerImportMetaUrl.ts
+++ b/packages/vite/src/node/plugins/workerImportMetaUrl.ts
@@ -7,6 +7,7 @@ import type { Plugin } from '../plugin'
 import { evalValue, injectQuery, transformStableResult } from '../utils'
 import type { ResolveFn } from '..'
 import { cleanUrl, slash } from '../../shared/utils'
+import { getDepsOptimizer, optimizedDepInfoFromFile } from '../optimizer'
 import type { WorkerType } from './worker'
 import { WORKER_FILE_ID, workerFileToUrl } from './worker'
 import { fileToUrl } from './asset'
@@ -114,6 +115,22 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
     asSrc: true,
   }
 
+  function tryOptimizedDepResolve(ssr: boolean, url: string, depId: string) {
+    const depsOptimizer = getDepsOptimizer(config, ssr)
+
+    if (depsOptimizer?.isOptimizedDepFile(depId)) {
+      const depFile = cleanUrl(depId)
+      const info = optimizedDepInfoFromFile(depsOptimizer.metadata, depFile)
+      const depSrc = info?.src
+      if (depSrc) {
+        const resolvedFile = path.resolve(path.dirname(depSrc), url)
+        return tryFsResolve(resolvedFile, fsResolveOptions)
+      }
+    }
+
+    return undefined
+  }
+
   return {
     name: 'vite:worker-import-meta-url',
 
@@ -148,10 +165,14 @@ export function workerImportMetaUrlPlugin(config: ResolvedConfig): Plugin {
           s ||= new MagicString(code)
           const workerType = getWorkerType(code, cleanString, endIndex)
           const url = rawUrl.slice(1, -1)
+
           let file: string | undefined
           if (url[0] === '.') {
             file = path.resolve(path.dirname(id), url)
-            file = tryFsResolve(file, fsResolveOptions) ?? file
+            file =
+              tryFsResolve(file, fsResolveOptions) ??
+              tryOptimizedDepResolve(options?.ssr === true, url, id) ??
+              file
           } else {
             workerResolver ??= config.createResolver({
               extensions: [],