|17:00-18:30 |Round Table: Get to know each other (on zoom) |
| When | What | Who |
|---|---|---|
| 09:00-10:00 | Lecture: Overview of different single-cell platforms and assays | Susan Kloet |
| 10:00-10:30 | break | |
| 10:30-12:00 | Lecture: scRNA-seq platforms | Miao-Ping Chien |
| 12:00-13:00 | lunch | |
| 13:00-14:00 | Lecture: Preprocessing | Roberta Menafra |
| 14:00-14:30 | break | |
| 14:30-15:30 | Lecture: Quality control and normalization | Ahmed Mahfouz |
| 15:30-15:45 | break | |
| 15:45-17:00 | Practical: Quality control and normalization | TAs (Chiel Wevers, Hina Nazkhan, Remco Hoogenboezem) |
| When | What | Who |
|---|---|---|
| 09:00-10:00 | Lecture: Experimental design & sample preparation | Susan Kloet |
| 10:00-10:30 | break | |
| 10:30-11:30 | Lecture: Single-cell multi-omics | Miao-Ping Chien |
| 11:30-13:00 | lunch | |
| 13:00-14:00 | Lecture: Dimensionality reduction | Thomas Höllt |
| 14:00-14:30 | break | |
| 14:30-16:00 | Practical: Dimensionality reduction | TAs (Chiel Wevers, Hina Nazkhan, Gregory van Beek) |
| When | What | Who |
|---|---|---|
| 09:00-10:00 | Lecture: Clustering and cell annotation | Lieke Michielsen |
| 10:00-10:30 | break | |
| 10:30-12:00 | Practical: Clustering and cell annotation | TAs (Chiel Wevers, Remco Hoogenboezem, Gregory van Beek) |
| 12:00-13:00 | lunch | |
| 13:00-14:00 | Lecture: Data integration | Tamim Abdelaal |
| 14:00-14:30 | break | |
| 14:30-16:00 | Practical: Data integration | TAs (Chiel Wevers, Remco Hoogenboezem, Gregory van Beek) |
| 16:00-17:00 | Lecture: Spatial Transcriptomics | Anna Alemany |
| When | What | Who |
|---|---|---|
| 09:00-10:00 | Lecture: Trajectory inference | Mohammed Charrout |
| 10:00-10:30 | break | |
| 10:30-12:00 | Practical: Trajectory inference | TAs (Hina Nazkhan, Remco Hoogenboezem, Gregory van Beek) |
| 12:00-13:00 | lunch | |
| 13:00-14:00 | Lecture: Differential expression analysis | Ahmed Mahfouz |
| 14:00-14:30 | break | |
| 14:30-16:00 | Practical: Differential expression analysis | TAs (Hina Nazkhan, Remco Hoogenboezem, Gregory van Beek) |
Note: this takes place on zoom
| When | What | Who |
|---|---|---|
| 09:00-09:45 | Dissecting intratumoral T cell states in human cancer using single cell technologies | Anne van der Leun (NKI) |
| 09:45-10:30 | Converting transient progenitors to stem cells | Micha Drukker (LACDR) |
| 10:30-11:00 | break | |
| 11:00-11:45 | Longitudinal single-cell epitope and RNA-sequencing reveals the immunological impact of type 1 interferon autoantibodies in critical COVID-19 | Monique van der Wijst (UMCG) |
| 11:45-12:30 | Drug response and tolerance in normal and cancer cells | Klaas Mulder (RIMLS) |