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Change scale settings on MST #26

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bspurgeon opened this issue Jun 19, 2019 · 0 comments
Open

Change scale settings on MST #26

bspurgeon opened this issue Jun 19, 2019 · 0 comments

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@bspurgeon
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Using the FlowSOM plugin on FlowJo, I've generated MSTs for two sample types (both colored on a single marker of interest).

Both samples express the marker at different levels, but the MSTs are scaled and colored individually (one from ~200-550; the other from ~200-650). Accordingly, it is impossible to tell the difference between the samples by comparing color intensity alone (i.e., the min/max intensities are different, but they are both colored the same shades of blue/red).

Does anyone know if (and how) it is possible to modify the scale settings so they are the same (e.g., ~200-650) on both MSTs?

Any guidance would be gratefully accepted.

Best,

Ben

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