diff --git a/src/adafruit_blinka/board/rp2040_one_u2if.py b/src/adafruit_blinka/board/rp2040_one_u2if.py
new file mode 100644
index 00000000..5379fd18
--- /dev/null
+++ b/src/adafruit_blinka/board/rp2040_one_u2if.py
@@ -0,0 +1,61 @@
+# SPDX-FileCopyrightText: 2021 Melissa LeBlanc-Williams for Adafruit Industries
+#
+# SPDX-License-Identifier: MIT
+"""
+Pin definitions for the Waveshare RP2040 One with u2if firmware.
+
+Adafruit CircuitPython; Waveshare RP2040 One with rp2040
+>>> import board
+>>> board.
+A0              A1              A2              A3
+D2              D3              D10             D11
+D12             D13             D14             D15
+D17             D18             D19             D20
+D21             D22             D23             D24
+D25             D9              I2C             MISO
+MOSI            NEOPIXEL        RX              SCK
+SCL             SDA             SPI             TX
+UART
+"""
+
+from adafruit_blinka.microcontroller.rp2040_u2if import pin
+
+D2 = pin.GP2
+D3 = pin.GP3
+D9 = pin.GP9
+D10 = pin.GP10
+D11 = pin.GP11
+D12 = pin.GP12
+D13 = pin.GP13
+D14 = pin.GP14
+D15 = pin.GP15
+D17 = pin.GP17
+D18 = pin.GP18
+D19 = pin.GP19
+D20 = pin.GP20
+D21 = pin.GP21
+D22 = pin.GP22
+D23 = pin.GP23
+D24 = pin.GP24
+D25 = pin.GP25
+
+A0 = pin.GP26
+A1 = pin.GP27
+A2 = pin.GP28
+# A3 = pin.GP29 # not currently supported in firmware
+
+NEOPIXEL = pin.GP16
+
+TX = pin.GP0
+RX = pin.GP1
+
+SCL = pin.GP5
+SDA = pin.GP4
+
+SCLK = SCK = pin.GP6
+MOSI = pin.GP7
+MISO = pin.GP8
+
+# access u2if via pin instance to open for specifc VID/PID
+# pylint:disable = protected-access
+pin.GP0._u2if_open_hid(0x2E8A, 0x103A)
diff --git a/src/board.py b/src/board.py
index e8a58166..8105fd41 100644
--- a/src/board.py
+++ b/src/board.py
@@ -404,6 +404,9 @@
 elif board_id == ap_board.WALNUT_PI_1B:
     from adafruit_blinka.board.walnutpi.walnutpi1b import *
 
+elif board_id == ap_board.RP2040_ONE_U2IF:
+    from adafruit_blinka.board.rp2040_one_u2if import *
+
 elif board_id == ap_board.OS_AGNOSTIC_BOARD:
     from adafruit_blinka.board.generic_agnostic_board import *