diff --git a/pkg/agent/agent_windows.go b/pkg/agent/agent_windows.go
index d17fd821779..3ecb469e307 100644
--- a/pkg/agent/agent_windows.go
+++ b/pkg/agent/agent_windows.go
@@ -43,6 +43,10 @@ func (i *Initializer) prepareHostNetwork() error {
 		}
 		// Save the uplink adapter name to check if the OVS uplink port has been created in prepareOVSBridge stage.
 		i.nodeConfig.UplinkNetConfig.Name = hnsNetwork.NetworkAdapterName
+
+		// Save the uplink adapter MAC to modify Pod traffic source MAC if the packet is directly output to the uplink
+		// interface in OVS pipeline.
+		i.nodeConfig.UplinkNetConfig.MAC, _ = net.ParseMAC(hnsNetwork.SourceMac)
 		return nil
 	}
 	if _, ok := err.(hcsshim.NetworkNotFoundError); !ok {
diff --git a/pkg/agent/openflow/pipeline.go b/pkg/agent/openflow/pipeline.go
index e082b43b61d..449532a1980 100644
--- a/pkg/agent/openflow/pipeline.go
+++ b/pkg/agent/openflow/pipeline.go
@@ -1357,6 +1357,7 @@ func (f *featurePodConnectivity) l3FwdFlowToRemoteViaUplink(remoteGatewayMAC net
 			MatchProtocol(ipProtocol).
 			MatchRegMark(NotAntreaFlexibleIPAMRegMark).
 			MatchDstIPNet(peerSubnet).
+			Action().SetSrcMAC(f.nodeConfig.UplinkNetConfig.MAC).
 			Action().SetDstMAC(remoteGatewayMAC).
 			Action().GotoTable(L3DecTTLTable.GetID()).
 			Done()
diff --git a/pkg/agent/openflow/pipeline_windows.go b/pkg/agent/openflow/pipeline_windows.go
index 486911d26ae..59c6dbe54ac 100644
--- a/pkg/agent/openflow/pipeline_windows.go
+++ b/pkg/agent/openflow/pipeline_windows.go
@@ -85,6 +85,7 @@ func (f *featurePodConnectivity) l3FwdFlowToRemoteViaRouting(localGatewayMAC net
 				MatchDstIP(peerIP).
 				MatchCTStateRpl(true).
 				MatchCTStateTrk(true).
+				Action().SetSrcMAC(f.nodeConfig.UplinkNetConfig.MAC).
 				Action().SetDstMAC(remoteGatewayMAC).
 				Action().NextTable().
 				Done(),