diff --git a/RESOURCES/FEATURE_FLAGS.md b/RESOURCES/FEATURE_FLAGS.md
index d029ca6c3cbd6..da0c50deb6733 100644
--- a/RESOURCES/FEATURE_FLAGS.md
+++ b/RESOURCES/FEATURE_FLAGS.md
@@ -91,7 +91,6 @@ These features flags currently default to True and **will be removed in a future
 - DASHBOARD_FILTERS_EXPERIMENTAL
 - DASHBOARD_NATIVE_FILTERS
 - DASHBOARD_NATIVE_FILTERS_SET
-- DISABLE_DATASET_SOURCE_EDIT
 - ENABLE_EXPLORE_DRAG_AND_DROP
 - ENABLE_EXPLORE_JSON_CSRF_PROTECTION
 - ENABLE_TEMPLATE_REMOVE_FILTERS
diff --git a/UPDATING.md b/UPDATING.md
index b5c48924b727e..cd2ea0804137b 100644
--- a/UPDATING.md
+++ b/UPDATING.md
@@ -29,6 +29,8 @@ assists people when migrating to a new version.
 
 ### Breaking Changes
 
+- [26331](https://github.com/apache/superset/issues/26331): Removes the deprecated `DISABLE_DATASET_SOURCE_EDIT` feature flag. The previous value of the feature flag was `False` and now the feature is permanently removed.
+
 ### Potential Downtime
 
 ### Other
diff --git a/superset-frontend/packages/superset-ui-core/src/utils/featureFlags.ts b/superset-frontend/packages/superset-ui-core/src/utils/featureFlags.ts
index 6bc77e0e87a1a..e3ad0a5d126d6 100644
--- a/superset-frontend/packages/superset-ui-core/src/utils/featureFlags.ts
+++ b/superset-frontend/packages/superset-ui-core/src/utils/featureFlags.ts
@@ -34,7 +34,6 @@ export enum FeatureFlag {
   DASHBOARD_VIRTUALIZATION = 'DASHBOARD_VIRTUALIZATION',
   DASHBOARD_RBAC = 'DASHBOARD_RBAC',
   DATAPANEL_CLOSED_BY_DEFAULT = 'DATAPANEL_CLOSED_BY_DEFAULT',
-  DISABLE_DATASET_SOURCE_EDIT = 'DISABLE_DATASET_SOURCE_EDIT',
   DISABLE_LEGACY_DATASOURCE_EDITOR = 'DISABLE_LEGACY_DATASOURCE_EDITOR',
   DRILL_TO_DETAIL = 'DRILL_TO_DETAIL',
   DRILL_BY = 'DRILL_BY',
diff --git a/superset-frontend/src/components/Datasource/DatasourceEditor.jsx b/superset-frontend/src/components/Datasource/DatasourceEditor.jsx
index 751001297a92a..e4a4d0116a098 100644
--- a/superset-frontend/src/components/Datasource/DatasourceEditor.jsx
+++ b/superset-frontend/src/components/Datasource/DatasourceEditor.jsx
@@ -629,9 +629,6 @@ class DatasourceEditor extends React.PureComponent {
     this.setColumns = this.setColumns.bind(this);
     this.validateAndChange = this.validateAndChange.bind(this);
     this.handleTabSelect = this.handleTabSelect.bind(this);
-    this.allowEditSource = !isFeatureEnabled(
-      FeatureFlag.DISABLE_DATASET_SOURCE_EDIT,
-    );
     this.currencies = ensureIsArray(props.currencies).map(currencyCode => ({
       value: currencyCode,
       label: `${getCurrencySymbol({
@@ -1050,23 +1047,21 @@ class DatasourceEditor extends React.PureComponent {
     const { datasource } = this.state;
     return (
       <div>
-        {this.allowEditSource && (
-          <EditLockContainer>
-            <span role="button" tabIndex={0} onClick={this.onChangeEditMode}>
-              {this.state.isEditMode ? (
-                <Icons.LockUnlocked iconColor={theme.colors.grayscale.base} />
-              ) : (
-                <Icons.LockLocked iconColor={theme.colors.grayscale.base} />
-              )}
-            </span>
-            {!this.state.isEditMode && (
-              <div>{t('Click the lock to make changes.')}</div>
-            )}
-            {this.state.isEditMode && (
-              <div>{t('Click the lock to prevent further changes.')}</div>
+        <EditLockContainer>
+          <span role="button" tabIndex={0} onClick={this.onChangeEditMode}>
+            {this.state.isEditMode ? (
+              <Icons.LockUnlocked iconColor={theme.colors.grayscale.base} />
+            ) : (
+              <Icons.LockLocked iconColor={theme.colors.grayscale.base} />
             )}
-          </EditLockContainer>
-        )}
+          </span>
+          {!this.state.isEditMode && (
+            <div>{t('Click the lock to make changes.')}</div>
+          )}
+          {this.state.isEditMode && (
+            <div>{t('Click the lock to prevent further changes.')}</div>
+          )}
+        </EditLockContainer>
         <div className="m-l-10 m-t-20 m-b-10">
           {DATASOURCE_TYPES_ARR.map(type => (
             <Radio
diff --git a/superset-frontend/src/components/Datasource/DatasourceEditor.test.jsx b/superset-frontend/src/components/Datasource/DatasourceEditor.test.jsx
index 9100eb6c86b2d..9a3d8455808c9 100644
--- a/superset-frontend/src/components/Datasource/DatasourceEditor.test.jsx
+++ b/superset-frontend/src/components/Datasource/DatasourceEditor.test.jsx
@@ -190,22 +190,6 @@ describe('DatasourceEditor', () => {
       expect(virtualRadioBtn).toBeDisabled();
     });
   });
-
-  describe('render editor with feature flag false', () => {
-    beforeAll(() => {
-      isFeatureEnabledMock = jest
-        .spyOn(uiCore, 'isFeatureEnabled')
-        .mockImplementation(() => true);
-    });
-
-    it('disable edit Source tab', async () => {
-      await asyncRender(props);
-      expect(
-        screen.queryByRole('img', { name: /lock-locked/i }),
-      ).not.toBeInTheDocument();
-      isFeatureEnabledMock.mockRestore();
-    });
-  });
 });
 
 describe('DatasourceEditor RTL', () => {
diff --git a/superset/config.py b/superset/config.py
index 348baef5454af..37ed0004fec94 100644
--- a/superset/config.py
+++ b/superset/config.py
@@ -404,7 +404,6 @@ class D3Format(TypedDict, total=False):
 DEFAULT_FEATURE_FLAGS: dict[str, bool] = {
     # Experimental feature introducing a client (browser) cache
     "CLIENT_CACHE": False,  # deprecated
-    "DISABLE_DATASET_SOURCE_EDIT": False,  # deprecated
     # When using a recent version of Druid that supports JOINs turn this on
     "DRUID_JOINS": False,
     "DYNAMIC_PLUGINS": False,
diff --git a/superset/views/base.py b/superset/views/base.py
index 9149c7ad919ac..db9ee645afc14 100644
--- a/superset/views/base.py
+++ b/superset/views/base.py
@@ -88,7 +88,6 @@
     "SUPERSET_DASHBOARD_POSITION_DATA_LIMIT",
     "SUPERSET_DASHBOARD_PERIODICAL_REFRESH_LIMIT",
     "SUPERSET_DASHBOARD_PERIODICAL_REFRESH_WARNING_MESSAGE",
-    "DISABLE_DATASET_SOURCE_EDIT",
     "ENABLE_JAVASCRIPT_CONTROLS",
     "DEFAULT_SQLLAB_LIMIT",
     "DEFAULT_VIZ_TYPE",