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evaluate using chipseq qc template for cutandrun output #51

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abartlett004 opened this issue Oct 11, 2024 · 0 comments
Open

evaluate using chipseq qc template for cutandrun output #51

abartlett004 opened this issue Oct 11, 2024 · 0 comments

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@abartlett004
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Input for chipseq qc and whether it is available in cutandrun output:

  1. coldata_fn: yes, use nfcore samplesheet

  2. multiqc_data_dir:

  • multiqc_fastqc.txt: yes, needed for total reads
  • multiqc_samtools_stats.txt: yes, needed for mapped reads, mapped reads pct
  • multiqc_phantompeakqualtools.txt: no. needed for nsc, rsc, but cutandrun doesnt run this tool. maybe can calculate another way?
  • multiqc_frip_score-plot.txt: no. needed for frip, but maybe can calculate another way? confusing that this is absent because nf-core output documentation suggests there should be a FRiP score section in their multiqc report. maybe absent because of processes that we skipped?
  • multiqc_peak_count-plot.txt: yes, but named multiqc_primary_peakcounts_plot.txt
  • mqc_picard_deduplication_1.txt: yes, needed to calculate nrf
  1. peaks_dir: yes

  2. counts_fn: no. there are no deseq2 processes run in this pipeline, nor any other processes that would fragment the genome into bins and calculate counts

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