diff --git a/sdk/python/feast/errors.py b/sdk/python/feast/errors.py
index f8a288940a..01e8ecbaca 100644
--- a/sdk/python/feast/errors.py
+++ b/sdk/python/feast/errors.py
@@ -204,6 +204,11 @@ def __init__(
         )
 
 
+class SavedDatasetLocationAlreadyExists(Exception):
+    def __init__(self, location: str):
+        super().__init__(f"Saved dataset location {location} already exists.")
+
+
 class FeastOfflineStoreInvalidName(Exception):
     def __init__(self, offline_store_class_name: str):
         super().__init__(
diff --git a/sdk/python/feast/feature_store.py b/sdk/python/feast/feature_store.py
index ea13c3a8db..02225a7b52 100644
--- a/sdk/python/feast/feature_store.py
+++ b/sdk/python/feast/feature_store.py
@@ -1146,6 +1146,7 @@ def create_saved_dataset(
         storage: SavedDatasetStorage,
         tags: Optional[Dict[str, str]] = None,
         feature_service: Optional[FeatureService] = None,
+        allow_overwrite: bool = False,
     ) -> SavedDataset:
         """
         Execute provided retrieval job and persist its outcome in given storage.
@@ -1154,6 +1155,14 @@ def create_saved_dataset(
         Name for the saved dataset should be unique within project, since it's possible to overwrite previously stored dataset
         with the same name.
 
+        Args:
+            from_: The retrieval job whose result should be persisted.
+            name: The name of the saved dataset.
+            storage: The saved dataset storage object indicating where the result should be persisted.
+            tags (optional): A dictionary of key-value pairs to store arbitrary metadata.
+            feature_service (optional): The feature service that should be associated with this saved dataset.
+            allow_overwrite (optional): If True, the persisted result can overwrite an existing table or file.
+
         Returns:
             SavedDataset object with attached RetrievalJob
 
@@ -1186,7 +1195,7 @@ def create_saved_dataset(
         dataset.min_event_timestamp = from_.metadata.min_event_timestamp
         dataset.max_event_timestamp = from_.metadata.max_event_timestamp
 
-        from_.persist(storage)
+        from_.persist(storage=storage, allow_overwrite=allow_overwrite)
 
         dataset = dataset.with_retrieval_job(
             self._get_provider().retrieve_saved_dataset(
diff --git a/sdk/python/feast/infra/offline_stores/bigquery.py b/sdk/python/feast/infra/offline_stores/bigquery.py
index da19bff5ec..5c3535071a 100644
--- a/sdk/python/feast/infra/offline_stores/bigquery.py
+++ b/sdk/python/feast/infra/offline_stores/bigquery.py
@@ -493,7 +493,7 @@ def _execute_query(
         block_until_done(client=self.client, bq_job=bq_job, timeout=timeout)
         return bq_job
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetBigQueryStorage)
 
         self.to_bigquery(
diff --git a/sdk/python/feast/infra/offline_stores/contrib/athena_offline_store/athena.py b/sdk/python/feast/infra/offline_stores/contrib/athena_offline_store/athena.py
index d7f11fb39f..92e133d02e 100644
--- a/sdk/python/feast/infra/offline_stores/contrib/athena_offline_store/athena.py
+++ b/sdk/python/feast/infra/offline_stores/contrib/athena_offline_store/athena.py
@@ -402,7 +402,7 @@ def _to_arrow_internal(self) -> pa.Table:
     def metadata(self) -> Optional[RetrievalMetadata]:
         return self._metadata
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetAthenaStorage)
         self.to_athena(table_name=storage.athena_options.table)
 
diff --git a/sdk/python/feast/infra/offline_stores/contrib/postgres_offline_store/postgres.py b/sdk/python/feast/infra/offline_stores/contrib/postgres_offline_store/postgres.py
index 1347f8b37c..80b1e089a1 100644
--- a/sdk/python/feast/infra/offline_stores/contrib/postgres_offline_store/postgres.py
+++ b/sdk/python/feast/infra/offline_stores/contrib/postgres_offline_store/postgres.py
@@ -297,7 +297,7 @@ def _to_arrow_internal(self) -> pa.Table:
     def metadata(self) -> Optional[RetrievalMetadata]:
         return self._metadata
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetPostgreSQLStorage)
 
         df_to_postgres_table(
diff --git a/sdk/python/feast/infra/offline_stores/contrib/spark_offline_store/spark.py b/sdk/python/feast/infra/offline_stores/contrib/spark_offline_store/spark.py
index 26d414232f..7c19b1e4e3 100644
--- a/sdk/python/feast/infra/offline_stores/contrib/spark_offline_store/spark.py
+++ b/sdk/python/feast/infra/offline_stores/contrib/spark_offline_store/spark.py
@@ -275,7 +275,7 @@ def _to_arrow_internal(self) -> pyarrow.Table:
             self.to_spark_df().write.parquet(temp_dir, mode="overwrite")
             return pq.read_table(temp_dir)
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         """
         Run the retrieval and persist the results in the same offline store used for read.
         Please note the persisting is done only within the scope of the spark session.
diff --git a/sdk/python/feast/infra/offline_stores/contrib/trino_offline_store/trino.py b/sdk/python/feast/infra/offline_stores/contrib/trino_offline_store/trino.py
index b5f0b1f950..5a3a9737d3 100644
--- a/sdk/python/feast/infra/offline_stores/contrib/trino_offline_store/trino.py
+++ b/sdk/python/feast/infra/offline_stores/contrib/trino_offline_store/trino.py
@@ -126,7 +126,7 @@ def to_trino(
         self._client.execute_query(query_text=query)
         return destination_table
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         """
         Run the retrieval and persist the results in the same offline store used for read.
         """
diff --git a/sdk/python/feast/infra/offline_stores/file.py b/sdk/python/feast/infra/offline_stores/file.py
index ca945c3ff3..742366d42e 100644
--- a/sdk/python/feast/infra/offline_stores/file.py
+++ b/sdk/python/feast/infra/offline_stores/file.py
@@ -1,3 +1,4 @@
+import os
 import uuid
 from datetime import datetime
 from pathlib import Path
@@ -11,13 +12,16 @@
 import pytz
 from pydantic.typing import Literal
 
-from feast import FileSource, OnDemandFeatureView
 from feast.data_source import DataSource
-from feast.errors import FeastJoinKeysDuringMaterialization
+from feast.errors import (
+    FeastJoinKeysDuringMaterialization,
+    SavedDatasetLocationAlreadyExists,
+)
 from feast.feature_logging import LoggingConfig, LoggingSource
 from feast.feature_view import DUMMY_ENTITY_ID, DUMMY_ENTITY_VAL, FeatureView
 from feast.infra.offline_stores.file_source import (
     FileLoggingDestination,
+    FileSource,
     SavedDatasetFileStorage,
 )
 from feast.infra.offline_stores.offline_store import (
@@ -30,6 +34,7 @@
     get_pyarrow_schema_from_batch_source,
 )
 from feast.infra.registry.base_registry import BaseRegistry
+from feast.on_demand_feature_view import OnDemandFeatureView
 from feast.repo_config import FeastConfigBaseModel, RepoConfig
 from feast.saved_dataset import SavedDatasetStorage
 from feast.usage import log_exceptions_and_usage
@@ -83,8 +88,13 @@ def _to_arrow_internal(self):
         df = self.evaluation_function().compute()
         return pyarrow.Table.from_pandas(df)
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetFileStorage)
+
+        # Check if the specified location already exists.
+        if not allow_overwrite and os.path.exists(storage.file_options.uri):
+            raise SavedDatasetLocationAlreadyExists(location=storage.file_options.uri)
+
         filesystem, path = FileSource.create_filesystem_and_path(
             storage.file_options.uri,
             storage.file_options.s3_endpoint_override,
diff --git a/sdk/python/feast/infra/offline_stores/file_source.py b/sdk/python/feast/infra/offline_stores/file_source.py
index 52a687a52c..135409ed04 100644
--- a/sdk/python/feast/infra/offline_stores/file_source.py
+++ b/sdk/python/feast/infra/offline_stores/file_source.py
@@ -96,12 +96,20 @@ def __eq__(self, other):
         )
 
     @property
-    def path(self):
-        """
-        Returns the path of this file data source.
-        """
+    def path(self) -> str:
+        """Returns the path of this file data source."""
         return self.file_options.uri
 
+    @property
+    def file_format(self) -> Optional[FileFormat]:
+        """Returns the file format of this file data source."""
+        return self.file_options.file_format
+
+    @property
+    def s3_endpoint_override(self) -> Optional[str]:
+        """Returns the s3 endpoint override of this file data source."""
+        return self.file_options.s3_endpoint_override
+
     @staticmethod
     def from_proto(data_source: DataSourceProto):
         return FileSource(
@@ -177,24 +185,33 @@ def get_table_query_string(self) -> str:
 class FileOptions:
     """
     Configuration options for a file data source.
+
+    Attributes:
+        uri: File source url, e.g. s3:// or local file.
+        s3_endpoint_override: Custom s3 endpoint (used only with s3 uri).
+        file_format: File source format, e.g. parquet.
     """
 
+    uri: str
+    file_format: Optional[FileFormat]
+    s3_endpoint_override: str
+
     def __init__(
         self,
+        uri: str,
         file_format: Optional[FileFormat],
         s3_endpoint_override: Optional[str],
-        uri: Optional[str],
     ):
         """
         Initializes a FileOptions object.
 
         Args:
+            uri: File source url, e.g. s3:// or local file.
             file_format (optional): File source format, e.g. parquet.
             s3_endpoint_override (optional): Custom s3 endpoint (used only with s3 uri).
-            uri (optional): File source url, e.g. s3:// or local file.
         """
+        self.uri = uri
         self.file_format = file_format
-        self.uri = uri or ""
         self.s3_endpoint_override = s3_endpoint_override or ""
 
     @classmethod
@@ -269,6 +286,17 @@ def to_data_source(self) -> DataSource:
             s3_endpoint_override=self.file_options.s3_endpoint_override,
         )
 
+    @staticmethod
+    def from_data_source(data_source: DataSource) -> "SavedDatasetStorage":
+        assert isinstance(data_source, FileSource)
+        return SavedDatasetFileStorage(
+            path=data_source.path,
+            file_format=data_source.file_format
+            if data_source.file_format
+            else ParquetFormat(),
+            s3_endpoint_override=data_source.s3_endpoint_override,
+        )
+
 
 class FileLoggingDestination(LoggingDestination):
     _proto_kind = "file_destination"
diff --git a/sdk/python/feast/infra/offline_stores/offline_store.py b/sdk/python/feast/infra/offline_stores/offline_store.py
index 741b97e2fd..b3b17eaed3 100644
--- a/sdk/python/feast/infra/offline_stores/offline_store.py
+++ b/sdk/python/feast/infra/offline_stores/offline_store.py
@@ -173,8 +173,16 @@ def on_demand_feature_views(self) -> List[OnDemandFeatureView]:
         pass
 
     @abstractmethod
-    def persist(self, storage: SavedDatasetStorage):
-        """Synchronously executes the underlying query and persists the result in the same offline store."""
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
+        """
+        Synchronously executes the underlying query and persists the result in the same offline store
+        at the specified destination.
+
+        Args:
+            storage: The saved dataset storage object specifying where the result should be persisted.
+            allow_overwrite: If True, a pre-existing location (e.g. table or file) can be overwritten.
+                Currently not all individual offline store implementations make use of this parameter.
+        """
         pass
 
     @property
diff --git a/sdk/python/feast/infra/offline_stores/redshift.py b/sdk/python/feast/infra/offline_stores/redshift.py
index 2acf06017d..1c20ff0c5a 100644
--- a/sdk/python/feast/infra/offline_stores/redshift.py
+++ b/sdk/python/feast/infra/offline_stores/redshift.py
@@ -483,7 +483,7 @@ def to_redshift(self, table_name: str) -> None:
                 query,
             )
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetRedshiftStorage)
         self.to_redshift(table_name=storage.redshift_options.table)
 
diff --git a/sdk/python/feast/infra/offline_stores/snowflake.py b/sdk/python/feast/infra/offline_stores/snowflake.py
index 98db97b179..8239aec34c 100644
--- a/sdk/python/feast/infra/offline_stores/snowflake.py
+++ b/sdk/python/feast/infra/offline_stores/snowflake.py
@@ -460,7 +460,7 @@ def to_arrow_chunks(self, arrow_options: Optional[Dict] = None) -> Optional[List
 
         return arrow_batches
 
-    def persist(self, storage: SavedDatasetStorage):
+    def persist(self, storage: SavedDatasetStorage, allow_overwrite: bool = False):
         assert isinstance(storage, SavedDatasetSnowflakeStorage)
         self.to_snowflake(table_name=storage.snowflake_options.table)
 
diff --git a/sdk/python/feast/saved_dataset.py b/sdk/python/feast/saved_dataset.py
index e2004d15f4..4a3043a873 100644
--- a/sdk/python/feast/saved_dataset.py
+++ b/sdk/python/feast/saved_dataset.py
@@ -8,6 +8,7 @@
 
 from feast.data_source import DataSource
 from feast.dqm.profilers.profiler import Profile, Profiler
+from feast.importer import import_class
 from feast.protos.feast.core.SavedDataset_pb2 import SavedDataset as SavedDatasetProto
 from feast.protos.feast.core.SavedDataset_pb2 import SavedDatasetMeta, SavedDatasetSpec
 from feast.protos.feast.core.SavedDataset_pb2 import (
@@ -31,6 +32,16 @@ def __new__(cls, name, bases, dct):
         return kls
 
 
+_DATA_SOURCE_TO_SAVED_DATASET_STORAGE = {
+    "FileSource": "feast.infra.offline_stores.file_source.SavedDatasetFileStorage",
+}
+
+
+def get_saved_dataset_storage_class_from_path(saved_dataset_storage_path: str):
+    module_name, class_name = saved_dataset_storage_path.rsplit(".", 1)
+    return import_class(module_name, class_name, "SavedDatasetStorage")
+
+
 class SavedDatasetStorage(metaclass=_StorageRegistry):
     _proto_attr_name: str
 
@@ -43,11 +54,24 @@ def from_proto(storage_proto: SavedDatasetStorageProto) -> "SavedDatasetStorage"
 
     @abstractmethod
     def to_proto(self) -> SavedDatasetStorageProto:
-        ...
+        pass
 
     @abstractmethod
     def to_data_source(self) -> DataSource:
-        ...
+        pass
+
+    @staticmethod
+    def from_data_source(data_source: DataSource) -> "SavedDatasetStorage":
+        data_source_type = type(data_source).__name__
+        if data_source_type in _DATA_SOURCE_TO_SAVED_DATASET_STORAGE:
+            cls = get_saved_dataset_storage_class_from_path(
+                _DATA_SOURCE_TO_SAVED_DATASET_STORAGE[data_source_type]
+            )
+            return cls.from_data_source(data_source)
+        else:
+            raise ValueError(
+                f"This method currently does not support {data_source_type}."
+            )
 
 
 class SavedDataset:
diff --git a/sdk/python/tests/integration/e2e/test_validation.py b/sdk/python/tests/integration/e2e/test_validation.py
index 26b46d9648..771061b206 100644
--- a/sdk/python/tests/integration/e2e/test_validation.py
+++ b/sdk/python/tests/integration/e2e/test_validation.py
@@ -65,6 +65,7 @@ def test_historical_retrieval_with_validation(environment, universal_data_source
         from_=reference_job,
         name="my_training_dataset",
         storage=environment.data_source_creator.create_saved_dataset_destination(),
+        allow_overwrite=True,
     )
     saved_dataset = store.get_saved_dataset("my_training_dataset")
 
@@ -95,6 +96,7 @@ def test_historical_retrieval_fails_on_validation(environment, universal_data_so
         from_=reference_job,
         name="my_other_dataset",
         storage=environment.data_source_creator.create_saved_dataset_destination(),
+        allow_overwrite=True,
     )
 
     job = store.get_historical_features(
@@ -172,6 +174,7 @@ def test_logged_features_validation(environment, universal_data_sources):
         ),
         name="reference_for_validating_logged_features",
         storage=environment.data_source_creator.create_saved_dataset_destination(),
+        allow_overwrite=True,
     )
 
     log_source_df = store.get_historical_features(
@@ -245,6 +248,7 @@ def test_e2e_validation_via_cli(environment, universal_data_sources):
         from_=retrieval_job,
         name="reference_for_validating_logged_features",
         storage=environment.data_source_creator.create_saved_dataset_destination(),
+        allow_overwrite=True,
     )
     reference = saved_dataset.as_reference(
         name="test_reference", profiler=configurable_profiler
diff --git a/sdk/python/tests/integration/offline_store/test_persist.py b/sdk/python/tests/integration/offline_store/test_persist.py
new file mode 100644
index 0000000000..8e6f182917
--- /dev/null
+++ b/sdk/python/tests/integration/offline_store/test_persist.py
@@ -0,0 +1,54 @@
+import pytest
+
+from feast.errors import SavedDatasetLocationAlreadyExists
+from feast.saved_dataset import SavedDatasetStorage
+from tests.integration.feature_repos.repo_configuration import (
+    construct_universal_feature_views,
+)
+from tests.integration.feature_repos.universal.entities import (
+    customer,
+    driver,
+    location,
+)
+
+
+@pytest.mark.integration
+@pytest.mark.universal_offline_stores(only=["file"])
+def test_persist_does_not_overwrite(environment, universal_data_sources):
+    """
+    Tests that the persist method does not overwrite an existing location in the offline store.
+
+    This test currently is only run against the file offline store as it is the only implementation
+    that prevents overwriting. As more offline stores add this check, they should be added to this test.
+    """
+    store = environment.feature_store
+    entities, datasets, data_sources = universal_data_sources
+    feature_views = construct_universal_feature_views(data_sources)
+    store.apply([driver(), customer(), location(), *feature_views.values()])
+
+    features = [
+        "customer_profile:current_balance",
+        "customer_profile:avg_passenger_count",
+        "customer_profile:lifetime_trip_count",
+    ]
+
+    entity_df = datasets.entity_df.drop(
+        columns=["order_id", "origin_id", "destination_id"]
+    )
+    job = store.get_historical_features(
+        entity_df=entity_df,
+        features=features,
+    )
+
+    with pytest.raises(SavedDatasetLocationAlreadyExists):
+        # Copy data source destination to a saved dataset destination.
+        saved_dataset_destination = SavedDatasetStorage.from_data_source(
+            data_sources.customer
+        )
+
+        # This should fail since persisting to a preexisting location is not allowed.
+        store.create_saved_dataset(
+            from_=job,
+            name="my_training_dataset",
+            storage=saved_dataset_destination,
+        )
diff --git a/sdk/python/tests/integration/offline_store/test_universal_historical_retrieval.py b/sdk/python/tests/integration/offline_store/test_universal_historical_retrieval.py
index cd61822e1c..73c5152d47 100644
--- a/sdk/python/tests/integration/offline_store/test_universal_historical_retrieval.py
+++ b/sdk/python/tests/integration/offline_store/test_universal_historical_retrieval.py
@@ -381,6 +381,7 @@ def test_historical_features_persisting(
         name="saved_dataset",
         storage=environment.data_source_creator.create_saved_dataset_destination(),
         tags={"env": "test"},
+        allow_overwrite=True,
     )
 
     event_timestamp = DEFAULT_ENTITY_DF_EVENT_TIMESTAMP_COL