diff --git a/scene/2d/animated_sprite_2d.cpp b/scene/2d/animated_sprite_2d.cpp
index 4734f97e235a..b33c2f43d4ac 100644
--- a/scene/2d/animated_sprite_2d.cpp
+++ b/scene/2d/animated_sprite_2d.cpp
@@ -388,6 +388,7 @@ void AnimatedSprite2D::play(const StringName &p_animation, const bool p_backward
 		}
 	}
 
+	is_over = false;
 	set_playing(true);
 }