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Thank you very much for the CB2 package, it is great and provides us with the quantification of the sgRNA in a very quick and easy way.
When running run_sgrna_quant it output the correct count matrix in regards to the ref. sgRNA names. But the sequences associated with the ref. sgRNA are incorrect. It seems to arise from the C++ fucntion quant. It returns Rcpp::_["sequence"] = ref.seq , which somehow is not working. Replacing ref.seq with sgRNA_hash hopefully fixes the problem.
I am unsure about the fix but hopefully, you can find an easy solution to return the correct sequences associated with the ref. sgRNA names.
The text was updated successfully, but these errors were encountered:
Hi @DaneseAnna, thanks for reporting this. Yes, you are correct, and I made a quick fix. You are able to install CB2 using devtools, and I will submit the new version to CRAN, but it will take time. Let me know if you have any issue. I will close the issue once you confirm that it gets fixed.
Thank you very much for the CB2 package, it is great and provides us with the quantification of the sgRNA in a very quick and easy way.
When running
run_sgrna_quant
it output the correct count matrix in regards to the ref. sgRNA names. But the sequences associated with the ref. sgRNA are incorrect. It seems to arise from the C++ fucntionquant
. It returnsRcpp::_["sequence"] = ref.seq
, which somehow is not working. Replacing ref.seq with sgRNA_hash hopefully fixes the problem.I am unsure about the fix but hopefully, you can find an easy solution to return the correct sequences associated with the ref. sgRNA names.
The text was updated successfully, but these errors were encountered: