diff --git a/CHANGELOG.md b/CHANGELOG.md
index f397eca124a6..e54ea1cb2619 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -4,6 +4,7 @@
 
 ### Fixes
 
+- `[jest-config]` Treat `setupFilesAfterEnv` like `setupFiles` when normalizing configs against presets ([#9495](https://github.com/facebook/jest/pull/9495))
 - `[jest-snapshot]` Downgrade semver to v6 to support node 8 ([#9451](https://github.com/facebook/jest/pull/9451))
 - `[jest-transform]` Correct sourcemap behavior for transformed and instrumented code ([#9460](https://github.com/facebook/jest/pull/9460))
 - `[pretty-format]` Export `OldPlugin` type ([#9491](https://github.com/facebook/jest/pull/9491))
diff --git a/packages/jest-config/src/__tests__/normalize.test.js b/packages/jest-config/src/__tests__/normalize.test.js
index 26bf21bb3a8d..b3b59a894df8 100644
--- a/packages/jest-config/src/__tests__/normalize.test.js
+++ b/packages/jest-config/src/__tests__/normalize.test.js
@@ -1137,6 +1137,7 @@ describe('preset', () => {
         preset: 'react-native',
         rootDir: '/root/path/foo',
         setupFiles: ['a'],
+        setupFilesAfterEnv: ['a'],
         transform: {a: 'a'},
       },
       {},
@@ -1151,6 +1152,10 @@ describe('preset', () => {
       '/node_modules/a',
       '/node_modules/b',
     ]);
+    expect(options.setupFilesAfterEnv.sort()).toEqual([
+      '/node_modules/a',
+      '/node_modules/b',
+    ]);
     expect(options.transform).toEqual([
       ['a', '/node_modules/a', {}],
       ['b', '/node_modules/b', {}],
@@ -1278,45 +1283,48 @@ describe('preset with globals', () => {
   });
 });
 
-describe('preset without setupFiles', () => {
-  let Resolver;
-  beforeEach(() => {
-    Resolver = require('jest-resolve');
-    Resolver.findNodeModule = jest.fn(
-      name => path.sep + 'node_modules' + path.sep + name,
-    );
-  });
+describe.each(['setupFiles', 'setupFilesAfterEnv'])(
+  'preset without %s',
+  configKey => {
+    let Resolver;
+    beforeEach(() => {
+      Resolver = require('jest-resolve');
+      Resolver.findNodeModule = jest.fn(
+        name => path.sep + 'node_modules' + path.sep + name,
+      );
+    });
 
-  beforeAll(() => {
-    jest.doMock(
-      '/node_modules/react-foo/jest-preset',
-      () => ({
-        moduleNameMapper: {b: 'b'},
-        modulePathIgnorePatterns: ['b'],
-      }),
-      {virtual: true},
-    );
-  });
+    beforeAll(() => {
+      jest.doMock(
+        '/node_modules/react-foo/jest-preset',
+        () => ({
+          moduleNameMapper: {b: 'b'},
+          modulePathIgnorePatterns: ['b'],
+        }),
+        {virtual: true},
+      );
+    });
 
-  afterAll(() => {
-    jest.dontMock('/node_modules/react-foo/jest-preset');
-  });
+    afterAll(() => {
+      jest.dontMock('/node_modules/react-foo/jest-preset');
+    });
 
-  it('should normalize setupFiles correctly', () => {
-    const {options} = normalize(
-      {
-        preset: 'react-foo',
-        rootDir: '/root/path/foo',
-        setupFiles: ['a'],
-      },
-      {},
-    );
+    it(`should normalize ${configKey} correctly`, () => {
+      const {options} = normalize(
+        {
+          [configKey]: ['a'],
+          preset: 'react-foo',
+          rootDir: '/root/path/foo',
+        },
+        {},
+      );
 
-    expect(options).toEqual(
-      expect.objectContaining({setupFiles: ['/node_modules/a']}),
-    );
-  });
-});
+      expect(options).toEqual(
+        expect.objectContaining({[configKey]: ['/node_modules/a']}),
+      );
+    });
+  },
+);
 
 describe('runner', () => {
   let Resolver;
diff --git a/packages/jest-config/src/normalize.ts b/packages/jest-config/src/normalize.ts
index f0cf0213eee0..a2454c373732 100644
--- a/packages/jest-config/src/normalize.ts
+++ b/packages/jest-config/src/normalize.ts
@@ -156,6 +156,11 @@ const setupPreset = (
   if (options.setupFiles) {
     options.setupFiles = (preset.setupFiles || []).concat(options.setupFiles);
   }
+  if (options.setupFilesAfterEnv) {
+    options.setupFilesAfterEnv = (preset.setupFilesAfterEnv || []).concat(
+      options.setupFilesAfterEnv,
+    );
+  }
   if (options.modulePathIgnorePatterns && preset.modulePathIgnorePatterns) {
     options.modulePathIgnorePatterns = preset.modulePathIgnorePatterns.concat(
       options.modulePathIgnorePatterns,