diff --git a/.changeset/breezy-plants-act.md b/.changeset/breezy-plants-act.md
new file mode 100644
index 000000000..d1c3673b0
--- /dev/null
+++ b/.changeset/breezy-plants-act.md
@@ -0,0 +1,5 @@
+---
+'myst-parser': patch
+---
+
+Remove .py from linkify domains
diff --git a/package-lock.json b/package-lock.json
index d121f350e..88f7f8f54 100644
--- a/package-lock.json
+++ b/package-lock.json
@@ -11787,6 +11787,14 @@
         "url": "https://github.com/sponsors/sindresorhus"
       }
     },
+    "node_modules/tlds": {
+      "version": "1.250.0",
+      "resolved": "https://registry.npmjs.org/tlds/-/tlds-1.250.0.tgz",
+      "integrity": "sha512-rWsBfFCWKrjM/o2Q1TTUeYQv6tHSd/umUutDjVs6taTuEgRDIreVYIBgWRWW4ot7jp6n0UVUuxhTLWBtUmPu/w==",
+      "bin": {
+        "tlds": "bin.js"
+      }
+    },
     "node_modules/tmp": {
       "version": "0.0.33",
       "resolved": "https://registry.npmjs.org/tmp/-/tmp-0.0.33.tgz",
@@ -14308,6 +14316,7 @@
         "myst-directives": "^1.0.22",
         "myst-roles": "^1.0.22",
         "myst-spec": "^0.0.5",
+        "tlds": "^1.250.0",
         "unified": "^10.1.1",
         "unist-builder": "^3.0.0",
         "unist-util-remove": "^3.1.0",
diff --git a/packages/myst-parser/package.json b/packages/myst-parser/package.json
index b3c0143b8..4074154f3 100644
--- a/packages/myst-parser/package.json
+++ b/packages/myst-parser/package.json
@@ -55,6 +55,7 @@
     "myst-directives": "^1.0.22",
     "myst-roles": "^1.0.22",
     "myst-spec": "^0.0.5",
+    "tlds": "^1.250.0",
     "unified": "^10.1.1",
     "unist-builder": "^3.0.0",
     "unist-util-remove": "^3.1.0",
diff --git a/packages/myst-parser/src/config.ts b/packages/myst-parser/src/config.ts
index 72f1e9f7b..664031b45 100644
--- a/packages/myst-parser/src/config.ts
+++ b/packages/myst-parser/src/config.ts
@@ -67,3 +67,6 @@ export const MARKDOWN_IT_CONFIG = {
     },
   },
 };
+
+// List of valid TLDs to exclude from linkify
+export const EXCLUDE_TLDS = ['py', 'md'];
diff --git a/packages/myst-parser/src/myst.ts b/packages/myst-parser/src/myst.ts
index c8c05085b..1bf82ad55 100644
--- a/packages/myst-parser/src/myst.ts
+++ b/packages/myst-parser/src/myst.ts
@@ -3,7 +3,8 @@ import { defaultDirectives } from 'myst-directives';
 import { defaultRoles } from 'myst-roles';
 import type { Plugin } from 'unified';
 import { VFile } from 'vfile';
-import { MARKDOWN_IT_CONFIG } from './config.js';
+import tlds from 'tlds';
+import { EXCLUDE_TLDS, MARKDOWN_IT_CONFIG } from './config.js';
 import { tokensToMyst } from './tokensToMyst.js';
 import {
   mathPlugin,
@@ -70,6 +71,9 @@ export function createTokenizer(opts?: Options) {
     } as any,
     markdownit,
   );
+  if (markdownit.linkify) {
+    tokenizer.linkify.tlds(tlds.filter((tld) => !EXCLUDE_TLDS.includes(tld)));
+  }
   if (extensions.smartquotes) tokenizer.enable('smartquotes');
   if (extensions.tables) tokenizer.enable('table');
   if (extensions.colonFences) tokenizer.use(colonFencePlugin);
diff --git a/packages/myst-parser/tests/linkify.spec.ts b/packages/myst-parser/tests/linkify.spec.ts
index 36b66c900..f6d460ffc 100644
--- a/packages/myst-parser/tests/linkify.spec.ts
+++ b/packages/myst-parser/tests/linkify.spec.ts
@@ -104,4 +104,18 @@ describe('linkify', () => {
       ],
     });
   });
+  it('dont linkify .py', () => {
+    const content = 'Link in paragraph: example.py';
+    const expected = {
+      type: 'root',
+      children: [
+        {
+          type: 'paragraph',
+          children: [{ type: 'text', value: 'Link in paragraph: example.py' }],
+        },
+      ],
+    };
+    expect(stripPositions(mystParse(content))).toEqual(expected);
+    expect(stripPositions(mystParse(content, { markdownit: { linkify: true } }))).toEqual(expected);
+  });
 });