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add optional flag for writing basecalled fast5s w/ guppy #20

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kapsakcj opened this issue Jun 15, 2020 · 2 comments
Open

add optional flag for writing basecalled fast5s w/ guppy #20

kapsakcj opened this issue Jun 15, 2020 · 2 comments
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enhancement New feature or request

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@kapsakcj
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This is so someone can run the basecalling script and have it write out basecalled fast5 files, for upload to NCBI/SRA

Something like -u for upload:

run_basecall-w-gpu.sh -i fast5s/ -o outdir/ -k rapid -b y -f r941 -u yes
@kapsakcj kapsakcj added the enhancement New feature or request label Jun 19, 2020
@kapsakcj
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Need to look into&test if guppy can produce both basecalled fast5s and fastqs at the same time or not

@kapsakcj
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ncbi prefers upload of demultiplexed fastqs, not fast5s. This may be unecessary....

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