diff --git a/UPGRADE.rst b/UPGRADE.rst
index ba488e1041e6..665821d4ef04 100644
--- a/UPGRADE.rst
+++ b/UPGRADE.rst
@@ -85,6 +85,19 @@ for example:
      wget https://packages.matrix.org/debian/pool/main/m/matrix-synapse-py3/matrix-synapse-py3_1.3.0+stretch1_amd64.deb
      dpkg -i matrix-synapse-py3_1.3.0+stretch1_amd64.deb
 
+Upgrading to v1.32.0
+====================
+
+Removal of old List Accounts Admin API
+--------------------------------------
+
+The deprecated v1 "list accounts" admin API (``GET /_synapse/admin/v1/users/<user_id>``) has been removed in this version.
+
+The `v2 list accounts API <https://github.com/matrix-org/synapse/blob/master/docs/admin_api/user_admin_api.rst#list-accounts>`_
+has been available since Synapse 1.7.0 (2019-12-13), and is accessible under ``GET /_synapse/admin/v2/users``.
+
+The deprecation of the old endpoint was announced with Synapse 1.28.0 (released on 2021-02-25).
+
 Upgrading to v1.29.0
 ====================
 
diff --git a/changelog.d/9401.removal b/changelog.d/9401.removal
new file mode 100644
index 000000000000..9c813e021574
--- /dev/null
+++ b/changelog.d/9401.removal
@@ -0,0 +1 @@
+Remove old admin API `GET /_synapse/admin/v1/users/<user_id>`.
\ No newline at end of file
diff --git a/synapse/rest/admin/__init__.py b/synapse/rest/admin/__init__.py
index 8457db1e2275..5daa795df1a4 100644
--- a/synapse/rest/admin/__init__.py
+++ b/synapse/rest/admin/__init__.py
@@ -62,7 +62,6 @@
     UserMembershipRestServlet,
     UserRegisterServlet,
     UserRestServletV2,
-    UsersRestServlet,
     UsersRestServletV2,
     UserTokenRestServlet,
     WhoisRestServlet,
@@ -248,7 +247,6 @@ def register_servlets_for_client_rest_resource(hs, http_server):
     PurgeHistoryStatusRestServlet(hs).register(http_server)
     DeactivateAccountRestServlet(hs).register(http_server)
     PurgeHistoryRestServlet(hs).register(http_server)
-    UsersRestServlet(hs).register(http_server)
     ResetPasswordRestServlet(hs).register(http_server)
     SearchUsersRestServlet(hs).register(http_server)
     ShutdownRoomRestServlet(hs).register(http_server)
diff --git a/synapse/rest/admin/users.py b/synapse/rest/admin/users.py
index fa7804583a31..595898c259fb 100644
--- a/synapse/rest/admin/users.py
+++ b/synapse/rest/admin/users.py
@@ -45,29 +45,6 @@
 logger = logging.getLogger(__name__)
 
 
-class UsersRestServlet(RestServlet):
-    PATTERNS = admin_patterns("/users/(?P<user_id>[^/]*)$")
-
-    def __init__(self, hs: "HomeServer"):
-        self.hs = hs
-        self.store = hs.get_datastore()
-        self.auth = hs.get_auth()
-        self.admin_handler = hs.get_admin_handler()
-
-    async def on_GET(
-        self, request: SynapseRequest, user_id: str
-    ) -> Tuple[int, List[JsonDict]]:
-        target_user = UserID.from_string(user_id)
-        await assert_requester_is_admin(self.auth, request)
-
-        if not self.hs.is_mine(target_user):
-            raise SynapseError(400, "Can only users a local user")
-
-        ret = await self.store.get_users()
-
-        return 200, ret
-
-
 class UsersRestServletV2(RestServlet):
     PATTERNS = admin_patterns("/users$", "v2")
 
diff --git a/tests/storage/test_client_ips.py b/tests/storage/test_client_ips.py
index 34e65260970e..a8a6ddc46610 100644
--- a/tests/storage/test_client_ips.py
+++ b/tests/storage/test_client_ips.py
@@ -390,7 +390,7 @@ def test_old_user_ips_pruned(self):
 class ClientIpAuthTestCase(unittest.HomeserverTestCase):
 
     servlets = [
-        synapse.rest.admin.register_servlets_for_client_rest_resource,
+        synapse.rest.admin.register_servlets,
         login.register_servlets,
     ]
 
@@ -434,7 +434,7 @@ def _runtest(self, headers, expected_ip, make_request_args):
             self.reactor,
             self.site,
             "GET",
-            "/_synapse/admin/v1/users/" + self.user_id,
+            "/_synapse/admin/v2/users/" + self.user_id,
             access_token=access_token,
             custom_headers=headers1.items(),
             **make_request_args,