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Add template for ChIP-seq assay #532

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allaway opened this issue Oct 16, 2024 · 2 comments
Open

Add template for ChIP-seq assay #532

allaway opened this issue Oct 16, 2024 · 2 comments
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bug Something isn't working priority: high

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@allaway
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allaway commented Oct 16, 2024

Describe the issue with the metadata
It would be helpful to have a chip-seq specific template. EpigeneticsSequencingAssay has content that is not relevant (e.g. bisulfiteConversionKitID) and is missing relevant content (e.g. assayTarget for the pulldown).

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@allaway allaway added the bug Something isn't working label Oct 16, 2024
@anngvu
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anngvu commented Nov 4, 2024

ChIP-seq specific template could also include antibody used. https://pmc.ncbi.nlm.nih.gov/articles/PMC3431496/#sec13

@changtotheintothemoon
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Hi Robert @allaway,

I found the section on Epigenetics sequencing assays here:
https://github.com/nf-osi/nf-metadata-dictionary/blob/main/modules/Template/Data.yaml#L271-L299

For ChIP-seq, here’s a list of possible types, though I'm not sure how many of these are relevant to NF projects. Please let me know if there are specific types we should focus on implementing:

  • Transcription Factor (TF) ChIP-seq
  • Polymerase ChIP-seq (e.g., RNA Pol II ChIP-seq)
  • Cohesin and CTCF ChIP-seq
  • Mediator Complex ChIP-seq
  • Histone Variant ChIP-seq (e.g., H2A.Z, H3.3)
  • Acetylation-Specific or Phosphorylation-Specific ChIP-seq
  • DNA-Binding Protein ChIP-seq (e.g., p300/CBP ChIP-seq)
  • Chromatin Remodeler ChIP-seq

Let me know your thoughts or any suggestions on implementing this. Thanks!

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