diff --git a/python/ray/_private/runtime_env/py_modules.py b/python/ray/_private/runtime_env/py_modules.py
index e8fa2bd8f754..947c08b6062f 100644
--- a/python/ray/_private/runtime_env/py_modules.py
+++ b/python/ray/_private/runtime_env/py_modules.py
@@ -2,6 +2,7 @@
 import os
 from types import ModuleType
 from typing import Any, Dict, Optional
+from pathlib import Path
 
 from ray.experimental.internal_kv import _internal_kv_initialized
 from ray._private.runtime_env.context import RuntimeEnvContext
@@ -47,6 +48,8 @@ def upload_py_modules_if_needed(
         if isinstance(module, str):
             # module_path is a local path or a URI.
             module_path = module
+        elif isinstance(module, Path):
+            module_path = str(module)
         elif isinstance(module, ModuleType):
             # NOTE(edoakes): Python allows some installed Python packages to
             # be split into multiple directories. We could probably handle
diff --git a/python/ray/_private/runtime_env/working_dir.py b/python/ray/_private/runtime_env/working_dir.py
index d134264c0932..140cadaa4b14 100644
--- a/python/ray/_private/runtime_env/working_dir.py
+++ b/python/ray/_private/runtime_env/working_dir.py
@@ -1,6 +1,7 @@
 import logging
 import os
 from typing import Any, Dict, Optional
+from pathlib import Path
 
 from ray._private.runtime_env.utils import RuntimeEnv
 from ray.experimental.internal_kv import _internal_kv_initialized
@@ -24,10 +25,13 @@ def upload_working_dir_if_needed(
     if working_dir is None:
         return runtime_env
 
-    if not isinstance(working_dir, str):
+    if not isinstance(working_dir, str) and not isinstance(working_dir, Path):
         raise TypeError(
-            "working_dir must be a string (either a local path or remote "
-            f"URI), got {type(working_dir)}.")
+            "working_dir must be a string or Path (either a local path "
+            f"or remote URI), got {type(working_dir)}.")
+
+    if isinstance(working_dir, Path):
+        working_dir = str(working_dir)
 
     # working_dir is already a URI -- just pass it through.
     try:
diff --git a/python/ray/tests/test_runtime_env_working_dir.py b/python/ray/tests/test_runtime_env_working_dir.py
index 350ec14d0f25..f3fd658a2441 100644
--- a/python/ray/tests/test_runtime_env_working_dir.py
+++ b/python/ray/tests/test_runtime_env_working_dir.py
@@ -46,7 +46,10 @@ def tmp_working_dir():
         yield tmp_dir
 
 
-@pytest.mark.parametrize("option", ["failure", "working_dir", "py_modules"])
+@pytest.mark.parametrize("option", [
+    "failure", "working_dir", "py_modules", "py_modules_path",
+    "working_dir_path"
+])
 @pytest.mark.skipif(sys.platform == "win32", reason="Fail to create temp dir.")
 def test_lazy_reads(start_cluster, tmp_working_dir, option: str):
     """Tests the case where we lazily read files or import inside a task/actor.
@@ -62,12 +65,21 @@ def call_ray_init():
             ray.init(address)
         elif option == "working_dir":
             ray.init(address, runtime_env={"working_dir": tmp_working_dir})
+        elif option == "working_dir_path":
+            ray.init(
+                address, runtime_env={"working_dir": Path(tmp_working_dir)})
         elif option == "py_modules":
             ray.init(
                 address,
                 runtime_env={
                     "py_modules": [str(Path(tmp_working_dir) / "test_module")]
                 })
+        elif option == "py_modules_path":
+            ray.init(
+                address,
+                runtime_env={
+                    "py_modules": [Path(tmp_working_dir) / "test_module"]
+                })
 
     call_ray_init()