diff --git a/trx/tests/test_io.py b/trx/tests/test_io.py
index 5a9dd8f..2f748d8 100644
--- a/trx/tests/test_io.py
+++ b/trx/tests/test_io.py
@@ -1,6 +1,7 @@
 #! /usr/bin/env python3
 # -*- coding: utf-8 -*-
 
+from copy import deepcopy
 import os
 
 import pytest
@@ -13,6 +14,7 @@
 except ImportError:
     dipy_available = False
 
+import trx.trx_file_memmap as tmm
 from trx.trx_file_memmap import TrxFile
 from trx.io import load, save, get_trx_tmpdir
 from trx.fetcher import (get_testing_files_dict,
@@ -77,3 +79,27 @@ def test_multi_load_save_rasmm(path):
         obj = load(out_path, os.path.join(dir, 'gs.nii'))
 
     assert_allclose(obj.streamlines._data, coord, rtol=1e-04, atol=1e-06)
+
+
+@pytest.mark.parametrize("path", [("gs.trx"), ("gs_fldr.trx")])
+@pytest.mark.skipif(not dipy_available, reason='Dipy is not installed.')
+def test_close_tmp_file(path):
+    dir = os.path.join(get_home(), 'gold_standard')
+    path = os.path.join(dir, path)
+
+    trx = tmm.load(path)
+    tmp_dir = deepcopy(trx._uncompressed_folder_handle)
+    sft = trx.to_sft()
+    trx.close()
+
+    coord_rasmm = np.loadtxt(os.path.join(get_home(), 'gold_standard',
+                                          'gs_rasmm_space.txt'))
+    coord_vox = np.loadtxt(os.path.join(get_home(), 'gold_standard',
+                                        'gs_vox_space.txt'))
+
+    # The folder trx representation does not need tmp files
+    if os.path.isfile(path):
+        assert not os.path.isdir(tmp_dir.name)
+    assert_allclose(sft.streamlines._data, coord_rasmm, rtol=1e-04, atol=1e-06)
+    sft.to_vox()
+    assert_allclose(sft.streamlines._data, coord_vox, rtol=1e-04, atol=1e-06)