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Graph iLISI gives a super low score for well-integrated data #418

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dariotommasini opened this issue Sep 8, 2024 · 0 comments
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@dariotommasini
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Hi scIB team,

Thank you for a great tool!

I had a question on ilisi_graph. I ran as such:

scib.me.ilisi_graph(sm.samap.adata, batch_key="batch", type_="knn")

I get a low number, indicative of poor batch mixing. Here is my kNN graph:

Screenshot 2024-09-07 at 5 54 54 PM

This graph is sorted by type, then by batch. This should be a good integration e.g. high cLISI and high iLISI. Can you think of a reason why this kNN graph would receive a low iLISI? It does indeed receive a high cLISI. Note that I used an integration method (SAMap) that deliberately lowers within-batch connectivities. However, even considering that, the neighborhood of each cell seems to include multiple species, but I get a iLISI score of 0.027. Thank you for the help!

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