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feat: add hg38 v45 reference genome #127

Merged
merged 6 commits into from
May 21, 2024
Merged

feat: add hg38 v45 reference genome #127

merged 6 commits into from
May 21, 2024

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kelly-sovacool
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@kelly-sovacool kelly-sovacool commented May 7, 2024

Changes

Adds configuration files for gencode release 45 of hg38.

Steps

Using PRI regions

build command

renee build \
    --sif-cache /data/CCBR_Pipeliner/SIFS \
    --ref-fa GRCh38.primary_assembly.genome.fa \
    --ref-name hg38 \
    --ref-gtf gencode.v45.primary_assembly.annotation.gtf \
    --gtf-ver 45 \
    --output /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45

change ownership

chown -R :CCBR_Pipeliner /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45
chmod -R a+rX /data/CCBR_Pipeliner/db/PipeDB/Indices/hg38_45

testing

biowulf ✅

/data/CCBR_Pipeliner/Pipelines/RENEE/renee-dev-sovacool/bin/renee run \
    --input /data/CCBR_Pipeliner/Pipelines/RENEE/develop/.tests/*.R1.fastq.gz \
    --genome hg38_45 \
    --mode slurm \
    --output /data/$USER/renee_test_gencode-45 \
    --sif-cache /data/CCBR_Pipeliner/SIFS

frce [in progress]

/mnt/projects/CCBR-Pipelines/pipelines/RENEE/renee-dev-sovacool/bin/renee run \
	--input latest/.tests/*.R?.fastq.gz \
	--output /scratch/cluster_scratch/$USER/renee_test_gencode-45 \
	--genome hg38_45 \
	--mode slurm \
	--tmp-dir /scratch/cluster_scratch/$USER \
	--sif-cache /mnt/projects/CCBR-Pipelines/SIFs

Issues

Resolves #126

PR Checklist

(Strikethrough any points that are not applicable.)

  • This comment contains a description of changes with justifications, with any relevant issues linked.
  • Update docs if there are any API changes.
  • Update CHANGELOG.md with a short description of any user-facing changes and reference the PR number. Guidelines: https://keepachangelog.com/en/1.1.0/

@kopardev kopardev added the RENEE RepoName label May 7, 2024
@kelly-sovacool kelly-sovacool marked this pull request as ready for review May 7, 2024 21:01
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kopardev commented May 7, 2024

Is fasta different from the one in /gpfs/gsfs10/users/CCBR_Pipeliner/Pipelines/RENEE/resources/fastas ?

@kelly-sovacool
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Is fasta different from the one in /gpfs/gsfs10/users/CCBR_Pipeliner/Pipelines/RENEE/resources/fastas ?

I had assumed they would be different, but they are actually the same. I'll switch it to use the existing one.

@kelly-sovacool
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this is ready @kopardev

@kopardev kopardev merged commit c5af68e into main May 21, 2024
3 checks passed
@kopardev kopardev deleted the gencode-45 branch May 21, 2024 01:51
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add support for v45 reference from gencode
2 participants