Method

Methods

This section contains information about the methods used in the project.

Data Processing

nf-core/rnaseq v3.14.0: Data was processed using nf-core/rnaseq v3.14.0 of the nf-core collection of workflows, utilising reproducible software environments from the Bioconda and Biocontainers projects.

Execution

The pipeline was executed with Nextflow v24.04.2 using the following command:

nextflow run nf-core/rnaseq --input scripts/samplesheet.csv --outdir results --genome BDGP6 -profile docker

Usage

This RNA-seq analysis pipeline employs STAR, RSEM, HISAT2, or Salmon for generating gene and isoform counts, as well as performing thorough quality control checks.