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Add better options for BDV in tutorial
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minnerbe authored Jun 21, 2023
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Expand Up @@ -131,9 +131,9 @@ For your dataset, the optimal choice of parameters may vary. A good baseline for
./st-align-global -c visium.n5 --absoluteThreshold 100 -sf 0.5 --lambda 0.0 --skipICP
```

7. <img align="right" src="https://github.com/PreibischLab/STIM/blob/master/src/main/resources/bdv-calm2-mbp-mtnd4.png" alt="Example alignment" width="240">The final dataset can for example be visualized and interactively explored using BigDataViewer. Therefore, we specify three genes `-g Calm2,Mbp,mt-Nd4`, a crisper rendering `-sf 0.6`, and a relative z-spacing between the two planes that shows them close to each other `-z 3`. Of course, the same data can be visualized using `st-explorer` and `st-render`.
7. <img align="right" src="https://github.com/PreibischLab/STIM/blob/master/src/main/resources/bdv-calm2-mbp-mtnd4.png" alt="Example alignment" width="240">The final dataset can for example be visualized and interactively explored using BigDataViewer. Therefore, we specify three genes `-g Calm2,Mbp,mt-Nd4`, a crisper rendering `-sf 0.5`, and a relative z-spacing between the two planes that shows them close to each other `-z 2`. Of course, the same data can be visualized using `st-explorer` and `st-render`, and visualization options such as color or contrast per gene can be adjusted manually.
```bash
./st-bdv-view -i visium.n5 -g Calm2,Mbp,mt-Nd4 -c '0,90' -sf 0.6 -z 3
./st-bdv-view -i visium.n5 -g Calm2,Mbp,mt-Nd4 -c '0,150' -sf 0.5 -z 2
```
We encourage you to use this small two slice dataset as a starting point for playing with and extending **STIM**. If you have any questions, feature requests or concerns please open an issue here on GitHub. Thanks so much!

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