UCPH's EvoGenomics section's Opentrons protocol library.
- Do NOT use the OT2 if you do not have the necessary training and have received the approval from Sarah Mak.
- Always use the OT2 LogBook to record usage.
- Use the Google Calendar to book OT2 robots. Ask Sarah Mak for details.
- Do NOT switch off the OT-2 unless there is a connection error (the power button is on the side).
- Do NOT press the button with BLUE light. It is just a light indicating whether the OT-2 is ready or not.
At EvoGenomics we have our own library of OT2 protocols developed and validated by internal users. All protocols are expected to contain:
- A python script with a descriptive name and version number specified (e.g. 2ml_to_96plate_v1.py).
- Procedures requiring multiple protocol setups are required to have a prefix number in name for indication of procedure stage.
- A readme file explaining the procedures to run the protocol (e.g. 2ml_to_96plate_v1.md)
- Some protocols (aliquoting, PCR mixes, etc.) might require csv files with basic information about sample positions, volumes, etc.
- Write the python code
- Validate the code in the Opentrons software
- Test the protocol without plasticware (only pipettes)
- Test the protocol with plasticware and dummy reagents
- Test the protocol with actual reagents
- temp_deck (if multiple modules [1-n])
- temp_plate (if multiple modules [1-n])
- mag_deck (if multiple modules [1-n])
- mag_plate (if multiple modules [1-n])
| Code | Description |
|---|---|
| s10 | 1-10 ul single channel pipette |
| s50 | 5-50 ul single channel pipette |
| s300 | 30-300 ul single channel pipette |
| s1000 | 100-1000 ul single channel pipette |
| m10 | 1-10 ul multichannel pipette |
| m50 | 5-50 ul multichannel pipette |
| m300 | 30-300 ul multichannel pipette |
