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Warning: the documentation on this wiki is deprecated and refers to V-pipe versions 1.0 and 2.0 (published in Bioinformatics). You can find the most recent documentation on running V-pipe 3.0 in the readme on the master branch.
For each sample, V-pipe produces several output files that are located in the corresponding sample-specific directory. First, the alignment file and consensus sequences are located in the alignments
and references
subdirectories, respectively. Second, output files containing SNVs and viral haplotypes are located in the variants
subdirectories.
Below, we provide an example of relevant output files and their locations, following the same structure as in the getting-started section. The output files for the two patient samples will be located in the following subdirectories:
working_directory
├─references
│ └───HXB2.fasta
└─samples
├──patient1
│ ├──20100113
│ │ ├──alignments
│ │ | └──REF_aln.bam
│ │ ├──references
| | | ├──ref_ambig.fasta
| | | └──ref_majority.fasta
| | └──variants
| | ├──SNVs
| | | └──snvs.vcf
| | └──global
| | └──contigs_stage_c.fasta
│ └──20110202
│ ├──alignments
│ | └──REF_aln.bam
│ ├──references
| | ├──ref_ambig.fasta
| | └──ref_majority.fasta
| └──variants
| ├──SNVs
| | └──snvs.vcf
| └──global
| └──contigs_stage_c.fasta
└──patient2
├──alignments
| └──REF_aln.bam
├──references
| ├──ref_ambig.fasta
| └──ref_majority.fasta
└──variants
├──SNVs
| └──snvs.vcf
└──global
└──contigs_stage_c.fasta
In addition, V-pipe generates a csv file containg the frequencies of all minor alleles that differ from the consensus among analysed samples. This ouput file is located in the variants
subdirectory
working_directory
└─variants
└───minority_variants.tsv