Skip to content

Commit

Permalink
Audit notebook all notebooks (#377)
Browse files Browse the repository at this point in the history
Co-authored-by: Emanuele Bezzi <[email protected]>
  • Loading branch information
pablo-gar and ebezzi authored Apr 18, 2023
1 parent 8f7af94 commit 0f49ea1
Show file tree
Hide file tree
Showing 10 changed files with 2,258 additions and 2,539 deletions.

Large diffs are not rendered by default.

Original file line number Diff line number Diff line change
@@ -1,27 +1,24 @@
{
"cells": [
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
"# Normalizing full-length gene sequencing data from the Census\n",
"# Normalizing full-length gene sequencing data\n",
"\n",
"The Census is a versioned container for the single-cell data hosted at [CELLxGENE Discover](https://cellxgene.cziscience.com/). The Census utilizes [SOMA](https://github.com/single-cell-data/SOMA/blob/main/abstract_specification.md) powered by [TileDB](https://tiledb.com/products/tiledb-embedded) for storing, accessing, and efficiently filtering data.\n",
"This tutorial shows you how to fetch full-length gene sequencing data from the Census and normalize it to account for gene length.\n",
"\n",
"This notebook shows you how to fetch full-length gene sequencing data from the Census and normalize it to account for gene length.\n",
"\n",
"**Contents**\n",
"\n",
"## Contents\n",
"\n",
"- Opening the census\n",
"- Fetching example full-length sequencing data (Smart-Seq2)\n",
"- Normalizing expression to account for gene length\n",
"- Validation through clustering analysis\n",
"1. Opening the census\n",
"2. Fetching example full-length sequencing data (Smart-Seq2)\n",
"3. Normalizing expression to account for gene length\n",
"4. Validation through clustering exploration\n",
"\n",
"## Opening the census\n",
"\n",
"First we open the Census, if you are not familiar with the basics of the Census API you should take a look at the notebook \"Learning about the CELLxGENE Census\" in `comp_bio_census_info.ipynb`."
"First we open the Census, if you are not familiar with the basics of the Census API you should take a look at notebook [Learning about the CZ CELLxGENE Census](https://cellxgene-census.readthedocs.io/en/latest/notebooks/analysis_demo/comp_bio_census_info.html)"
]
},
{
Expand All @@ -46,7 +43,6 @@
]
},
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
Expand All @@ -66,7 +62,6 @@
]
},
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
Expand Down Expand Up @@ -236,7 +231,6 @@
]
},
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
Expand All @@ -260,7 +254,6 @@
]
},
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
Expand Down Expand Up @@ -355,7 +348,6 @@
]
},
{
"attachments": {},
"cell_type": "markdown",
"metadata": {},
"source": [
Expand Down Expand Up @@ -455,7 +447,7 @@
"source": [
"All done! You can see that we now have real numbers instead of integers.\n",
"\n",
"## Validation through clustering analysis\n",
"## Validation through clustering exploration\n",
"\n",
"Let's perform some basic clustering analysis to see if cell types cluster as expected using the normalized counts.\n",
"\n",
Expand Down Expand Up @@ -621,7 +613,7 @@
"name": "python",
"nbconvert_exporter": "python",
"pygments_lexer": "ipython3",
"version": "3.10.6"
"version": "3.10.10"
},
"vscode": {
"interpreter": {
Expand Down
Loading

0 comments on commit 0f49ea1

Please sign in to comment.