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GACorrector

GACorrector是一个基于Python的程序,用于参考基因组和注释的更新。GACorrector的输入文件是待更新的参考基因组、待更新的参考基因组注释文件、用于更新的新基因序列和新基因的注释文件;输出文件是更新后的参考基因组和更新后的参考基因组注释文件。

GACorrector is a Python-based program used to update reference genomes and annotations. GACorrector takes a reference genome, genome annotation, sequences of new gene models and the annotations of new gene models as input; the output is an updated reference genome and and updated genome annotation file.

Tutorial

在Linux命令行执行:

In Linux command line, run the following command:

git clone https://github.com/huangyq89/GACorrector.git

进入../GACorrector/目录,执行以下命令解压tools.tar包:

Enter../GACorrector/directory,run the following command to decompresstools.tarpackage:

tar -xzvf tools.tar

打开测试文件所在目录../GACorrector/test/,在命令行执行以下命令:

Open the directory with test files../GACorrector/test/, run the following command:

python ../create_gtf.py test_sequence.fasta test_sequence_annotation.gtf processed_sequence.fasta

打开测试文件所在目录../GACorrector/test/,在命令行执行以下命令:

Open the directory with test files../GACorrector/test/, run the following command:

python ../GACorrector.py test_genome.fa test_annotation.gtf test_sequence.fasta  test_sequence_annotation.gtf output_genome.fa output_annotation.gtf processed_sequence.fasta

接收的参数依次为待更新的参考基因组待更新的注释文件create_gtf.py处理过的新基因序列新基因的注释文件更新后的参考基因组更新后的注释文件新基因原始序列

Parameters accepted are in order as follows:reference genome to be updatedgenome annotation to be updatednew gene models sequences processed by create_gtf.pyannotation of new gene modelsupdated reference genomeupdated genome annotationandraw sequences of new gene models.

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