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USDA-NRSP-8-gigas-rDNA

Pertinent documents

  1. Manuscript
  2. Proposal
  3. Tissue Sample List
  4. rDNA Github Issue
  5. DNA extraction protocol
  6. DNA extraction results
  7. Gannet Folder
  8. Nightingales Folder - F05/F14 prefix

Numbers

UW budget #: 63-7-one-one-7
Azenta PO #: EI1382037
Azenta Order tracking #: 30-835022638

Family names: F05, F12, F13, F14
Tissue storage location: 5-1-(3&4) in FTR -80C

Progress

  1. 20220728 - 4 Pacific Oyster families were generated by Pacific Hybreed and transfered to the Jamestown Point Whitney Shellfish Hatchery. Diploids and triploids were produced for each family using tetraploid crosses of parents.
  2. 20221114 - Whole body oyster tissue samples collected. n=15 per family per ploidy.
  3. 20220117 - Grace Leuchtenberger wrote up the DNA extraction protocol. Performed power analysis based on publicly available data. See notebook post
  4. 20230202 - DNA extractions with Grace L and Henry Berg.
  5. 20230314 - Shipped samples to Azenta (Short-read WGS, 30x coverage, 32 samples, 2 families, 8 samples per ploidy/family). See notebook post for more information.
  6. 20230329 - WGS results received from Azenta. Stored on nightingales. Intial QC and trimming done on Raven. Files a bit big for this platform (maxed out drive). See notebook post.

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