- Manuscript
- Proposal
- Tissue Sample List
- rDNA Github Issue
- DNA extraction protocol
- DNA extraction results
- Gannet Folder
- Nightingales Folder - F05/F14 prefix
UW budget #: 63-7-one-one-7
Azenta PO #: EI1382037
Azenta Order tracking #: 30-835022638
Family names: F05, F12, F13, F14
Tissue storage location: 5-1-(3&4) in FTR -80C
- 20220728 - 4 Pacific Oyster families were generated by Pacific Hybreed and transfered to the Jamestown Point Whitney Shellfish Hatchery. Diploids and triploids were produced for each family using tetraploid crosses of parents.
- 20221114 - Whole body oyster tissue samples collected. n=15 per family per ploidy.
- 20220117 - Grace Leuchtenberger wrote up the DNA extraction protocol. Performed power analysis based on publicly available data. See notebook post
- 20230202 - DNA extractions with Grace L and Henry Berg.
- 20230314 - Shipped samples to Azenta (Short-read WGS, 30x coverage, 32 samples, 2 families, 8 samples per ploidy/family). See notebook post for more information.
- 20230329 - WGS results received from Azenta. Stored on nightingales. Intial QC and trimming done on Raven. Files a bit big for this platform (maxed out drive). See notebook post.