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Update docs/usage/DEanalysis/rnaseq.md
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Co-authored-by: Matthias Hörtenhuber <[email protected]>
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LorenzoS96 and mashehu authored Nov 7, 2024
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The number of reads and the number of biological replicates are two critical factors that researchers need to carefully consider during the design of a RNA-seq experiment. While it may seem intuitive that having a large number of reads is always desirable, an excessive number can lead to unnecessary costs and computational burdens, without providing significant improvements in quality of the data. Instead, it is often more beneficial to prioritise the number of biological replicates, as it allows to capture the natural biological variation of the data. Biological replicates involve collecting and sequencing RNA from distinct biological samples (e.g., different individuals, tissues, or time points), helping to detect genuine changes in gene expression.

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This concept must not be confused with technical replicates that asses the technical variability of the sequencing platform by sequencing the same RNA sample multiple time.

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