- Follow instructions on GoSTRIPEs: https://github.com/BrendelGroup/GoSTRIPES/blob/master/HOWTO.md
- Clone GoSTRIPEs_sing to your system: git clone https://github.com/rtraborn/GoSTRIPES_sing
- Put fastqs and genome directory in your analysis directory as described in the above HOWTO.md. Specifically, add the fastq files (with the .fq suffix) to the downloads folder, and create a new genome folder inside the STRIPES/ directory.
- Clone the Singularity image in your STRIPES/ directory as follows:
singularity pull --name gostripes.simg shub://BrendelGroup/GoSTRIPES
. - Make a project directory for each replicate and cp
Makefile
into it. - Edit makefile (in STRIPES) as needed to run the analysis.
- Submit the job script launch_job.sh (edit the
source
line as needed and change headers if using non-TORQUE submission system.)
-
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A repo with the minimum amount of material needed for running GoSTRIPEs
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