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Problem with option gnuplotTested=true when calling canu #149

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guibar opened this issue Mar 14, 2019 · 4 comments
Open

Problem with option gnuplotTested=true when calling canu #149

guibar opened this issue Mar 14, 2019 · 4 comments

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@guibar
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guibar commented Mar 14, 2019

When running circlator with version 1.8 of canu, the execution fails because circlator call canu with the following command:

canu -useGrid=false gnuplotTested=true -assemble genomeSize=0.1m -d /root/lhs91/LHS91-pacbio/outcirc/03.assemble -p canu -pacbio-corrected /root/lhs91/LHS91-pacbio/outcirc/02.bam2reads.fastaq

and the parameter gnuplotTested=true is invalid for canu.
Is there a workaround for this?

Thanks,

@splaisan
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splaisan commented Mar 15, 2019

same here

The following command failed with exit code 1
canu -useGrid=false gnuplotTested=true -assemble genomeSize=0.1m 
-d /data2/gridion_data/2741/circlator_ont_canu-out/03.assemble 
-p canu -nanopore-corrected /data2/gridion_data/2741/circlator_ont_canu-out/02.bam2reads.fasta
ERROR:  Parameter 'gnuplotTested' is not known.

@maxgmarin
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Same here.

The following command that Circlator submitted fails for the same reason (Using Canu 1.8).

The following command failed with exit code 1
canu -useGrid=false gnuplotTested=true -assemble genomeSize=0.1m -d /n/data1/hms/dbmi/farhat/mm774/Projects/PacBio_Evaluation_Project/AnalysisPipelineOutput/PacmanPipeV2_ENA_T1/output/ERX3451130/pacbio/circlator/03.assemble -p canu -pacbio-corrected /n/data1/hms/dbmi/farhat/mm774/Projects/PacBio_Evaluation_Project/AnalysisPipelineOutput/PacmanPipeV2_ENA_T1/output/ERX3451130/pacbio/circlator/02.bam2reads.fasta

Giving the following error:

ERROR:  Parameter 'gnuplotTested' is not known.

@ilnamkang
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I experienced the same error and solved it by editing one file in the install directory.

The file is "assemble.py" located in "/usr/local/lib/python3.4/dist-packages/circlator" on my system.

Delete a line 'gnuplotTested=true' from the file. In my case, the line number was 86.

I wish this trick would work for others.

@EricDeveaud
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since canu 1.8 gnuplotTested disapeared see: from canu/1.8 Changelog

Option 'gnuplotTested' was removed. Failure to find or run gnuplot is now handled automagically. Issues #1084 and #1129.

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