This plugin provides two commands sourmash scripts mgsearch and
sourmash scripts mgmanysearch, that provide new & nicer outputs for
searching for genomes in metagenomes. It is a plugin for the sourmash
software.
Reporting the presence and estimating the abundance of queries in data sets is a core requirement of many bioinformatics analyses - metagenomics in particular.
This plugin provides two commands that use k-mers to estimate the presence and abundance of queries in data sets. Use cases include:
- detecting specific genomes in metagenomes;
- estimating the abundance of genomes in metagenomes;
- contig-level abundance estimation in metagenomes for binning;
- strain-level analysis of genomes in metagenomes;
The plugin uses FracMinHash-based estimation to calculate k-mer detection and estimate coverage based on k-mer multiplicity. These numbers correspond closely to mapping-based detection and coverage.
The plugin outputs Average Nucleotide Identity estimates using the approach described in Rahman Hera et al., 2023 and implemented in sourmash.
To install this plugin, run:
pip install sourmash_plugin_containment_search
(This will install sourmash if you do not already have it installed.)
This plugin enables two commands, mgsearch and mgmanysearch.
This command:
sourmash scripts mgsearch query.sig metagenome.sig [ metagenome2.sig ...] \
[ -o output.csv ]
will search for the query genome query.sig in one or more
metagenome.sig files, producing decent human-readable output and
(optionally) useful CSV outputs.
For example,
sourmash scripts mgsearch ../sourmash/podar-ref/0.fa.sig ../sourmash/SRR606249.trim.k31.sig.gz
produces:
Loaded query signature: CP001472.1 Acidobacterium capsulatum ATCC 51196, com...
p_genome avg_abund p_metag metagenome name
-------- --------- ------- ---------------
100.0% 55.4 3.1% SRR606249
This plugin will work with all the standard sourmash database types, too.
Note that the metagenomes must have been sketched with -p abund to
enable the avg_abund and p_metag columns.
This command:
sourmash scripts mgmanysearch --queries query1.sig [ query2.sig ... ]\
--against metagenome.sig [ metagenome2.sig ...] \
[ -o output.csv ]
will search for the queries query*.sig in one or more
metagenome*.sig files, producing decent human-readable output and
(optionally) useful CSV outputs.
sourmash search supports sample search x sample search, broadly -
perhaps too
broadly. And the output formats aren't that helpful.
sourmash prefetch supports metagenome overlap search against many
genomes, which is the reverse of this use case. Moreover,
prefetch doesn't provided weighted results
and its output isn't friendly.
sourmash gather has friendly and useful output, but can't be used to
calculate the overlap between a single query genome and many subject
metagenomes.
There is also some interest in reverse containment search.
The manysearch command of
the sourmash branchwater plugin
also does a nice containment search like this plugin, but it doesn't
provide nice human-readable output and it also doesn't provide
weighted results. (manysearch is, however, much lower memory &
probably a fair bit faster because it's mostly in Rust.)
This command is streaming, in the sense that it will load each metagenome, calculate the match, and then discard the metagenome. Hence its memory usage peaks with the largest metagenome, and its max should be driven by the size of the query + the size of the largest metagenome.
Each row contains the following information.
intersect_bp- overlap between genome and metagenome, estimated by multiplying the number of hashes by the scaled factor used.f_query- fraction of query (genome) found, aka "detection"; roughly matches the number of bases that will be covered by mapped metagenome reads.f_match- fraction of metagenome found, unweighted.f_match_weighted- fraction of metagenome found, weighted. Roughly matches the fraction of metagenome reads that will map to this genome.sum_weighted_found- sum of weights from intersecting hashes.average_abund- average abundance of weights intersecting hashes.median_abund- median abundance of weights from intersecting hashes.std_abund- std dev of weights from intersecting hashes.jaccard- (unweighted) Jaccard similarity between sketches.genome_containment_ani- ANI estimated from the genome containment in the metagenome. Use this for genome ANI estimates.match_containment_ani- ANI estimated from the metagenome containment in the genome.average_containment_ani- ANI estimated from the average of the genome and metagenome containments.max_containment_ani- ANI estimated from the max containment between genome/metagenome.potential_false_negative- True if the sketch size(s) were too small to give a reliable ANI estimate. False if ANI estimate is reliable.
ksize- ksize of comparison.moltype- moltype of comparison.scaled- scaled of comparison.
query_filename- genome filename from sketch.query_name- genome name.query_md5- genome md5.query_n_hashes- total number of hashes in the genome.
match_filename- metagenome filename from sketch.match_name- metagenome name.match_md5- metagenome md5.match_n_hashes- total number of hashes in the metagenome.match_n_weighted_hashes- total number of weighted hashes in metagenome.
We suggest filing issues in the main sourmash issue tracker as that receives more attention!
containment_search is developed at https://github.com/ctb/sourmash_plugin_containment_search.
Bump version number in pyproject.toml and push.
Make a new release on github.
Then pull, and:
python -m build
followed by twine upload dist/....