-
Notifications
You must be signed in to change notification settings - Fork 8
Installation and Dependencies
MiguelJulia edited this page Jun 7, 2019
·
5 revisions
PlasmidID needs all scripts located in /bin folder to be in the PATH. In order to do so, you need to add the following command to .bashrc file, normally located in /home/$USER/.bashrc:
export PATH=$PATH:/PATH/TO/PPLASMIDID/plasmidID/bin
In order to execute it, user needs to install all dependencies and execute plasmidID.sh with the required parameters.
In order to download plasmidID just clone the repository.
You will need the following software:
- Trimmomatic v0.33(Optional)
- Spades v3.8 (Optional)
- Perl v5.26.0
- NCBI_blast + v2.2.3
- Bedtools v2.25
- Bowtie 2 v2.2.4
- SAMtools v1.2
- prokka v1.12
- cd-hit v4.6.6
- circos v0.69.3
All these dependencies must be included in your $PATH for the pipeline to work without editing the code.
This pipeline has been run and tested with Centos 6.7